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Oncology Cytogenetics

The Oncology Cytogenetics service is a leading centre for high quality G-banded and fluorescence in-situ hybridisation (FISH) analysis of a wide range of malignant diseases.

Cytogenetic analysis can provide diagnostic and prognostic information critical to effective treatment decision making for oncology patients and relies on having the right diagnostic information from the best available technology.

An extensive portfolio of tests and a wide range of methodologies, coupled with high success rates and analytical and interpretive expertise, means we can offer a flexible service which meets the demands of oncology patient management.

Cytogenetic techniques

The conventional method of cytogenetic analysis involves processing cells from fresh tissue samples containing viable tumour cells in order to produce a G-banded karyotype.

FISH analysis targets specific genetic sequences; as it does not require dividing cells, it can be performed on a wider range of sample types, including formalin-fixed paraffin-embedded (FFPE) tissue sections and slides prepared using liquid-based cytology techniques.

Sample information

Liquid samples
  • Peripheral blood in lithium heparin or bone marrow transport medium. All other samples in bone marrow transport medium.
  • Do not spin down or freeze before sending.
  • Samples must arrive within 24 hours.
  • Samples not being dispatched the day they are taken should be refrigerated overnight at 4°C and sent the next morning.
  • Minimum volume - as available and dependant on white cell count.
  • Processed and stored as fixed cell suspensions. Retained for three years.
  • Slides used for G-banded karyotype analysis are stored indefinitely.

FFPE
  • 3-4µm sections on unstained APES slides with appropriate immunohistochemistry (IHC)/H&E slide for correlation.
  • Send sufficient slides to enable repeat testing if necessary.
  • Unused slides retained indefinitely; IHC/H&E slides will be returned to the referring centre when sample has been reported.

UroVysion FISH                
  • Unstained ThinPrep slide.

Reporting times:

Urgent: 95% of samples reported within 14 calendar days.
Routine: 95% of samples reported within 21 calendar days.

Test information

For disease specific test information, download our test guide. Tests can be ordered by completing the request form here and all samples should adhere to sample transport requirements. 

For key contacts and hours of operation visit the department key contacts page.